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KMID : 0385920120230050721
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Journal of the Korean Society of Emergency Medicine
2012 Volume.23 No. 5 p.721 ~ p.729
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Regulatory Effects of Fenofibrate with Inflammatory Response and Myocardiac Dysfunction in Lipopolysaccharidestimulated Heart Tissues
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Song Dong-Hoon
Hwang Yong
Yoo Su-Jin
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Abstract
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Purpose: This study was intended to establish experimental conditions for monitoring the cardioprotective effects of fenofibrate on cardiac function in lipopolysaccharide (LPS)- stimulated BalB/c mice.
Methods: To investigate the effects of fenofibrate on cardiac function, expression of Peroxisome proliferator-activated receptors (PPARs) and Peroxisome proliferator-activated receptor Gamma coactivator 1(PGC-1) and its target gene in the heart tissues of mice was compared after controls and LPS injection with pretreated fenofibrate or alone using reverse transcriptase-polymerase chain reaction (RTPCR), Western blot analysis, and immunohistochemistry. In addition, Enzyme-linked-immunosorbent-assays (ELISA) were performed for assessment of pro-inflammatory cytokines of blood serum.
Results: Pretreated with fenofibrate had protective effects of diminishing the levels of LPS-induced pro-inflammatory cytokines, including interleukin-1β(IL-1β), interleukin-6 (IL- 6), and tumor necrosis factor-α(TNF-α) and recovery from reduction of messenger Ribo-nucleic acid, protein level of PPARs and PGC-1 in LPS-administered heart tissue. In addition, increasing expression of PPARs and PGC-1 ameameliorated the expression and activity of catalase blocked production of lipid peroxidation.
Conclusion: Treatment with fenofibrate resulted in augmented expression of transcription factors and reduced production of pro-inflammatory cytokines and lipid peroxidation after LPS administration. Therefore, results of this study suggested that fenofibrate should not only have a protective effect but should also restore cardiac function in several cardiac dysfunctional situations.
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KEYWORD
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Sepsis, Lipopolysaccharide, Cardiac dysfunction, Fenofibrate
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